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Tuesday, 26 March 2019

DNA Extraction - Science

DNA Extraction of Kiwifruit

Our aim for today is to extract the DNA from some kiwifruit. We need to do the following:

Steps:
-Using a spatula, scoop 1/2 kiwifruit into a plastic (resealable) bag. Add 25ml water + a pinch of salt.
-Mush until a thick soup.
-Pour over cloth into a funnel into a beaker.
-Pour liquid into a test tube. Add dishwashing liquid + swirl. Allow it to sit for 5 minutes.
-Tilt test tube on a 45-degree angle and slowly pour 10ml of ethanol down the side.
-Allow it to sit.







These are the results. From the first one with no fluffy bubbles to the one above ("the fluffy bubbles" is the DNA ). This was the process of the DNA being extracted from the kiwifruit. It looked like the DNA began to float. 

Because water was added to the substance, it helped break the kiwifruit apart. The ethanol and dishwashing liquid helped to draw the DNA from the fruit.

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